In 2016, the appropriately named Toughie died in the Atlanta Botanical Garden. He was at least twelve years old, though his exact age is unknown. Toughie and another Rabb’s fringe-limbed tree frog were collected from Panama in 2005 for research on chytrid fungus, a deadly fungus that has been ravaging amphibian populations in the region. Amphibians, like Toughie, have the highest rate of endangerment, with a third of known species being at risk of extinction. Toughie became the face of the amphibian extinction crisis as visitors to his enclosure knew they were looking at the last of his kind.